Independent · non-commercial · publishes on a quarterly cycle|Current cycle 2026 Q3
Methodology|Interests|Funding|Corrections
Compound Evidence InstituteEvidence synthesis · established 2023Graded assessments of compounds, trials, methods and supply
Document set current to 30 July 2026
Defined term · Analytical chemistry

Freeze–thaw

Repeated freezing and thawing of a solution. Concentrates solutes in the shrinking liquid fraction, drives pH excursion as buffer components crystallise differentially, and promotes aggregation.

Document identifier
CEI-GT-161
Series
Defined term
Version
1.0
Published
30 Jul 2026
Last reviewed
30 Jul 2026
Certainty
Not rated

§1Freeze–thaw

§1.2Category

Analytical chemistry — Terms describing the determination of identity, purity, content and quality.

§1.3Related terms

  • Aggregation — Association of peptide molecules into higher-order species, driven by agitation, freeze–thaw cycling and proximity to the isoelectric point. Not detected by a…
  • In-use period — The time a reconstituted preparation may be stored and used, supported by stability-indicating data on that presentation. The Institute publishes no in-use claim for any…
Compound Evidence Institute · CEI-GT-161 · https://compoundevidence.com/glossary/freeze-thaw/ · retrieved 30 July 2026
Nothing published by the Institute is medical advice, a diagnosis, a prescription, a treatment recommendation or a purchasing recommendation. Compounds supplied for research use are not approved for human or veterinary use in any jurisdiction, and a favourable analytical assessment of a supplier is not a statement that any product is safe or effective. The Institute publishes certainty ratings and never recommendations. No telephone number, messaging handle or ordering channel appears anywhere on this site.